Effect of depletion of GPR30 on gene expression in female C57BL/6J mice

To test the cell-specific function of GPR30 in learning and memory, we generated global GPR30 knockout (KO) mice (delta3102), astrocyte-specific GPR30 KO (AG-KO) mice and neuron-specific GPR30 KO (NG-KO) mice. We found that GPR30 in astrocytes but not neurons is required to maintain normal learning and memory. To further elucidate the signaling pathway by which GPR30 plays a role in astrocyte function, high-throughput RNA sequencing of hippocampal tissues from delta3102, AG-KO, and NG-KO mice was performed. Comparative gene expression profiling analysis of RNA-seq data for WT, delta3102, AG-KO, and NG-KO mice为探究GPR30在学习与记忆中的细胞特异性功能，我们构建了全身性GPR30敲除（knockout, KO）小鼠（delta3102）、星形胶质细胞特异性GPR30敲除（AG-KO）小鼠以及神经元特异性GPR30敲除（NG-KO）小鼠。研究发现，星形胶质细胞中的GPR30而非神经元内的GPR30，是维持正常学习记忆功能的必要条件。为进一步阐明GPR30调控星形胶质细胞功能的信号通路，我们对delta3102、AG-KO及NG-KO小鼠的海马组织进行了高通量RNA测序。针对野生型（Wild Type, WT）、delta3102、AG-KO及NG-KO小鼠的RNA测序数据，我们开展了比较基因表达谱分析。