Genetic diversity of Moroccan date palm revealed by microsatellite markers

Abstract The genetic diversity between 23 Moroccan date palm cultivars collected from the National Palm Collection at the INRA (National Agricultural Research Institute) experimental field in Zagora was assessed using SSR markers that are specifically designed for date palm. Among the 16 tested SSR, 13 were successfully amplified, and were selected to carry out this study. 208 bands were amplified, ranging from 10 to 25 bands per cultivar with an average of 16 alleles per cultivar. The value of heterozygosity of the studied markers ranged from 0.11 to 0.30. The pairwise genetic distances between those cultivars ranged from 0.06 to 0.46. The hierarchical cluster analysis distributed the 23 genotypes into four different groups of one to ten cultivars.

摘要 本研究以采自摩洛哥扎古拉地区国家农业研究院（INRA, National Agricultural Research Institute）试验田国家椰枣种质资源库的23个摩洛哥椰枣栽培品种为材料，采用专为椰枣开发的简单序列重复（Simple Sequence Repeat，SSR）标记开展遗传多样性评估。在16个待测SSR标记中，13个成功完成扩增并被选为本研究的分析标记。本次实验共扩增得到208条条带，单品种扩增条带数介于10至25之间，平均每个品种含16个等位基因。所检测标记的杂合度取值范围为0.11至0.30。各栽培品种间的成对遗传距离介于0.06至0.46之间。层级聚类分析将23份基因型划分为4个不同类群，类群内栽培品种数量从1至10不等。