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Data for: A new reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay for DHAV-3 detection based on the POCKIT™ system

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doi.org2025-01-15 收录
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http://doi.org/10.17632/m82vhzkkbd.1
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These documents contain some important dates about the development and evaluation of this RT-iiPCR of DHAV-3. And we listed as follows: Fig 1 The linear relation of every dilution detected by rRT-PCR. Table 1 Reference strains and isolates used in this study for specificity testing of RT-iiPCR. Table 2 Primer information rRT-PCR assay. Table 3 Evaluation of the sensitivity of the rRT-PCR and RT-iiPCR assays to DHAV-3. Table 4 Performance evaluations of the PetNADTM and TRizol RNA extraction methods for the detection of DHAV-3 by rRT-PCR and RT-iiPCR, respectively. Table 5 The Contingency table for analysis of the level of agreement between the rRT-PCR and the RT-iiPCR assays for the detection of DHAV-3 in liver samples.

本数据集收录了关于DHAV-3 RT-iiPCR开发与评估过程中的若干重要日期。具体如下:图1展示了rRT-PCR检测到的每个稀释度的线性关系;表1列出了本研究中用于RT-iiPCR特异性测试的参考菌株和分离株;表2提供了rRT-PCR检测方法的引物信息;表3评估了rRT-PCR和RT-iiPCR检测DHAV-3灵敏度的性能;表4分别对PetNADTM和TRizol RNA提取方法在rRT-PCR和RT-iiPCR检测DHAV-3中的表现进行了性能评估;表5展示了rRT-PCR与RT-iiPCR检测肝脏样本中DHAV-3的一致性分析列联表。
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