Genome-wide identification of olfactory receptors and olfaction-mediated feed attractants in Japanese eel (Anguilla japonica) leptocephalus larvae

Supplementary Fig.1 (A) The sequence alignment of Homo sapiens RTP1S (AY562235.1), Mus musculus RTP1S (EU070411.1), Oryzias latipes RTP2 (XP_011479560.1), Oryzias latipes RTP3 (XP_004067938.1), Danio rerio RTP2 (NP_001373747.1), Anguilla japonica PTP3, and Anguilla japonica RTP4. (B) Comparison of OR52E8 expression on the cell-surface in HEK293T. Supplementary Fig.2 (A) Phylogenetic tree of Gαolf in Anguilla japonica, Mus musculus Gαolf(AY179169.1)and Homo sapiens Gαolf (AF493893.1). Data was resampled with 1,000 bootstrap replicates (see also Supplementary data5). (B) The sequence alignment of Anguilla japonica Gαolf1 Mus musculus Gαolf and Homo sapiens Gαolf. Supplementary Fig.3 The expressed localization of OR52E8 and OR52E4 in HEK293T. Supplementary Fig.4 (A) The 3D conformer of Lac and Trp. (B) Homology modeling of OR52E8 and OR52E4. (C) The CRE luciferase activity at different concentration of Lac and Trp. The data are presented as the mean ± SEM(n=3). Supplementary Data1. A phylogenetic tree of ORs from Japanese eel and three other teleost genomes. Supplementary Data2. The phylogenetic tree of the TAAR gene family. Supplementary Data3. The phylogenetic tree of the ORA gene family Supplementary Data4. Phylogenetic tree of Gαolf in Anguilla japonica, Mus musculus Gαolf(AY179169.1)and Homo sapiens Gαolf (AF493893.1). Supplementary Data5. The PubChem CIDs of the feed attractants and the results of the molecular docking. Supplementary Data6. Dual-luciferase assay data and one-way ANOVA results for OR52E4 in response to feed attractants.