SmcHD1,H3k27me3,CTCF and RAd21 ChIP-seq. SmcHD1,H3k27me3,CTCF and RAd21 ChIP-seq

In this study we investigate the role of the non-canonical SMC family protein, SmcHD1in the X inactivation. Overall design: Set of allele-specific ChIP-seq experiments on female clonal inter-specific (M.m.domesticus FVB x M.m.Castaneus) MEF cell lines: wild-type MEFs, SmcHD1 MomeD1 mut MEFs (SmcHD1 null) and SmcHD1 CRISPR KO MEFs (derived from wild-type MEFs after establishemnt of X inactivation). Each ChIP library was accompanied with respective input library and each experiment was done in biological replicates (2-4).