AHA 20CDA35310305 data

An intestine-specific <i>Scd1 </i>knockout model was developed by crossing <i>Scd1</i>^{<em>fl/fl</em>}<i> </i>mice with mice expressing Cre recombinase under the control of the Villin promoter to study the specific role of intestinal SCD1 in intestinal and whole-body lipid metabolism. The intestinal, hepatic and plasma lipid content and composition of these mice were evaluated by GC-MS analysis under chow fed and sucrose refed conditions. The role of intestinal SCD1 in the regulation of energy balance was also evaluated under chow fed and high-fat conditions. Bile acid content, composition, and signaling was analyzed. Additionally, metabolic phenotyping including body composition, indirect calorimetry and glucose tolerance analyses were conducted.

本研究通过将携带<i>Scd1</i>^fl/fl等位基因的小鼠与在Villin启动子调控下表达Cre重组酶的小鼠杂交，构建了肠道特异性硬脂酰辅酶A去饱和酶1（Scd1）敲除模型，以探究肠道SCD1在肠道及全身脂质代谢中的特异性功能。通过气相色谱-质谱联用（Gas Chromatography-Mass Spectrometry，GC-MS）分析，评估了普通饲料喂养与蔗糖再喂养条件下，该模型小鼠的肠道、肝脏及血浆脂质含量与组成；同时在普通饲料喂养及高脂喂养条件下，评估了肠道SCD1在能量平衡调控中的作用，并对胆汁酸的含量、组成及信号通路进行了分析。此外，还开展了包括体成分分析、间接测热法及糖耐量分析在内的代谢表型研究。