Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants

Additional data associated with the publication "Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants" Nature Plants, 2025. Abstract: The plant hormone auxin regulates a wide variety of transcriptional responses depending on the cell type, environment, and species. How this diversity is achieved may be related to the specific complement of auxin signalling components in each cell. The levels of activators (class-A AUXIN RESPONSE FACTORS) and repressors (class-B ARFs) are particularly important. Tight regulation of ARF protein levels is likely key in determining this balance. Through comparative analysis of novel, dominant mutants in maize and the moss Physcomitrium patens, we have discovered a ~500-million-year-old mechanism of class-B ARF protein level regulation mediated by proteasome degradation, important in determining cell fate decisions across land plants. Thus, our results add a key piece to the puzzle of how auxin regulates plant development. These datasets are in addition to those included in the supplmentary materials in the paper, the raw sequence data provided in the NCBI GEO deposit, and the github pages (https://github.com/ThePlantShapeLab/Truffula ) ## The raw sequence data associated with the project is available to download at GEO accession numbers GSE293375, GSE293431, GSE293432

本数据集关联发表于《Nature Plants》2025年的论文《Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants》（《对比突变分析揭示陆生植物中ARF调控的新机制》）。论文摘要：植物激素生长素（auxin）可根据细胞类型、环境条件及物种差异，调控多样化的转录响应过程。该响应多样性的产生机制，或与各细胞中生长素信号通路组分的特定组成密切相关。其中，激活因子（A型生长素响应因子AUXIN RESPONSE FACTORS，简称ARF）与抑制因子（B型ARF）的表达水平尤为关键。精准调控ARF蛋白丰度，或是维持该信号平衡的核心要素。本研究通过对玉米及小立碗藓（Physcomitrium patens）中新发现的显性突变体开展对比分析，揭示了一套起源于约5亿年前、由蛋白酶体降解介导的B型ARF蛋白水平调控机制，该机制对陆生植物的细胞命运决定具有重要意义。本研究结果为解析生长素调控植物发育的复杂谜题补充了关键一环。本数据集为论文补充材料、美国国家生物技术信息中心基因表达综合数据库（NCBI GEO）提交的原始测序数据以及GitHub页面（https://github.com/ThePlantShapeLab/Truffula）中已发布内容之外的附加数据集。本项目关联的原始测序数据可通过以下GEO登录号下载：GSE293375、GSE293431、GSE293432。