Participants Data F1000Research.xlsx

Obesity is a complex condition influenced by both genetic and environmental factors. While several studies have explored the genetic basis of obesity in diverse populations, little is known about its genetic underpinnings specifically in the Jordanian population. To address this gap, our study aims to unravel the genetic determinants of obesity among Jordanian individuals.Using a Genome-wide Association Study (GWAS) approach, we will investigate the occurrence and frequency of genetic variants associated with obesity in a cohort of 150 unrelated Jordanian adults. Participants will be categorized into three groups based on their body mass index (BMI): obese, overweight, and normal-weight subjects. Through high-resolution melt analysis (HRMA), we will genotype participants for specific single nucleotide polymorphisms (SNPs) known to be linked with obesity susceptibility genes, including rs2167270 in the LEP gene and rs1137100 in the LEPR gene.Our analysis will explore the association between these genetic variants and various phenotypic characteristics, including obesity risk, BMI, anthropometric measurements (weight, height), and metabolic parameters such as total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, and blood glucose levels.Preliminary results indicate a significant association between obesity risk and the rs2167270 mutation in the LEP gene, suggesting its potential role in predisposing individuals to obesity. Additionally, we observed marginal associations with BMI and glucose blood levels, as well as weak associations with cholesterol levels and the rs1137100 mutation in the LEPR gene.These findings shed light on the genetic factors contributing to obesity susceptibility and its related metabolic traits in the Jordanian population. Understanding the genetic architecture of obesity in this demographic could pave the way for personalized prevention and intervention strategies tailored to the Jordanian population. Further validation studies involving larger cohorts are essential to confirm and expand upon these initial findings.

肥胖是一种受遗传与环境因素共同调控的复杂病症。尽管已有多项研究在不同人群中探讨了肥胖的遗传基础，但针对约旦人群特有的肥胖遗传机制，目前仍所知有限。为填补这一研究空白，本研究旨在揭示约旦人群中肥胖的遗传决定因素。
本研究将采用全基因组关联研究（Genome-wide Association Study, GWAS）方法，对150名无亲缘关系的约旦成年人队列中与肥胖相关的遗传变异的存在及频率展开调查。研究对象将根据体质量指数（body mass index, BMI）分为三组：肥胖组、超重组与正常体重组。本研究将通过高分辨率熔解分析（high-resolution melt analysis, HRMA），对与肥胖易感基因相关的特定单核苷酸多态性（single nucleotide polymorphisms, SNPs）进行基因分型，包括LEP基因中的rs2167270以及LEPR基因中的rs1137100。
本研究将分析上述遗传变异与多种表型特征的关联，包括肥胖风险、体质量指数、人体测量指标（体重、身高）以及代谢参数（总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯与血糖水平）。
初步研究结果显示，LEP基因中的rs2167270突变与肥胖风险存在显著关联，提示该变异可能在个体肥胖易感性中发挥作用。此外，本研究还观察到该变异与体质量指数及血糖水平存在边际关联，与胆固醇水平及LEPR基因中的rs1137100突变则存在较弱关联。
上述研究结果揭示了约旦人群中影响肥胖易感性及其相关代谢特征的遗传因素。阐明该人群肥胖的遗传结构，可为针对约旦人群的个性化肥胖预防与干预策略提供依据。后续需开展更大队列的验证研究，以确认并拓展这些初步研究结果。