Study of LINC00458/HBL1 lncRNA expression during hiPSC differentiation to cardiomyocytes

Data were collected in order to assess the efficiency of hiPSC differentiation towards cardiomyocytes and the expression levels of lncRNAs during these differentiations. Moreover, the expression of splice isoforms of the lncRNA of interest was investigated. Deposited data encompass (1) raw (*.eds) and exported (*.xls) qPCR data, (2) raw (*.czi) confocal microscopy files, and (3) raw agarose gel images (*.jpg and *.tiff). (1) qPCR data were collected on a Quantstudio 6 Flex real-time PCR system (Thermo Fisher). (2) Images were collected using an Axio Observer Z1 inverted microscope (Carl Zeiss, Jena,Germany) equipped with the LSM 880 AiryScan confocal module with ZEN 2012 SP1 black edition software and processed in ImageJ Fiji (National Institutes of Health, Bethesda, MD). (3) Digital images of agarose gels were acquired using a ChemiDoc™ MP Imaging System (Bio-Rad) with Image Lab Touch Software (Bio-Rad).

本数据集旨在评估人诱导多能干细胞（human induced pluripotent stem cells, hiPSC）向心肌细胞的分化效率，以及该分化过程中长链非编码RNA（long non-coding RNAs, lncRNAs）的表达水平。此外，本研究还针对目标长链非编码RNA的可变剪接异构体的表达情况进行了分析。所提交的数据集包含以下三类数据：(1) 原始qPCR（quantitative real-time PCR, qPCR）数据（文件格式为*.eds）及导出的qPCR数据（文件格式为*.xls）；(2) 共聚焦显微镜原始成像文件（文件格式为*.czi）；(3) 琼脂糖凝胶电泳原始图像（文件格式为*.jpg及*.tiff）。各数据的采集与处理细节如下：(1) qPCR数据通过QuantStudio 6 Flex型实时荧光定量PCR系统（赛默飞世尔科技，Thermo Fisher）采集得到；(2) 成像数据使用搭载LSM 880 AiryScan共聚焦模块的Axio Observer Z1型倒置显微镜（德国耶拿卡尔蔡司公司，Carl Zeiss, Jena, Germany）采集，配套使用ZEN 2012 SP1黑色版软件完成图像采集，并通过ImageJ Fiji软件（美国国立卫生研究院，National Institutes of Health, 贝塞斯达，马里兰州）进行图像处理；(3) 琼脂糖凝胶电泳数字图像通过ChemiDoc™ MP成像系统（伯乐生命医学产品公司，Bio-Rad）及Image Lab Touch软件（Bio-Rad）采集获取。