ExtFig9A_RACE_PR8-HA_R1-R2-R3_LG290-292-293_annotated.tif

Xrn1 ko A549 cells were infected with WT or PR8-PA(∆X), or mock infected. RNA was extracted to run 5’ RACE using primers ~150-200 nt downstream of GCUG sites that are predicted to be inside a hairpin loop within viral mRNAs (in segment 4 or 8 as indicated)

将Xrn1敲除（knockout, ko）的A549细胞分别感染野生型（Wild Type, WT）或PR8-PA(ΔX)毒株，同时设置空白感染对照组。随后提取细胞RNA，采用距预测存在于病毒mRNA（对应第4或第8基因组节段，如标注所示）内发夹环结构内部的GCUG基序下游约150~200 nt的引物，进行5'末端快速扩增（Rapid Amplification of cDNA Ends, 5’ RACE）实验。