RNA-seq expression analysis of Pcf11 mutations Pcf11-13 and Pcf11-DNL

High Throughput bulk-sample RNA-sequencing expression data, was collected for triplicate samples of two distinct mutations of Pcf11 and a matched wildtype w303 samples under standard growth conditions. The overall goal was quantification of expression abundance and pre-mRNA processing changes in response to these mutations. RNA was prepared from three biological replicates of each mutant strain (Pcf11-13 and the novel mutant Pcf11-DNL, as described in the associated manuscript) and submitted to Genewiz for poly(A) selection and standard RNA sequencing using the Illumina HiSeq 3000 platform, with 2x150 bp paired end runs, and a return of 15-20 million read pairs per sample.