Single cell RNA-seq of forebrain cells isolated from wild-type mouse, rat, and chimeric mouse

We performed single cell RNA-seq to quantify gene expression in forebrain tissue from the wild-type mouse, rat, and chimeric mouse. Single cell data from WT mouse or rat was used to perform integrative analysis for identifying rat cells and mouse cells in the chimaeras, respectively.本研究针对野生型小鼠（wild-type mouse）、大鼠（rat）及嵌合小鼠（chimeric mouse）的前脑组织开展单细胞RNA测序（single cell RNA-seq），以定量检测其基因表达水平。分别采用野生型小鼠与野生型大鼠的单细胞测序数据，对该嵌合体中的大鼠细胞与小鼠细胞进行整合分析，以实现对应细胞的识别。