Dataset for: Expression of Striated Activator of Rho-Signaling (STARS) in human skeletal muscle following acute exercise and long-term training

Aim: The Striated Activator of Rho-Signaling (STARS) protein acts as a link between external stimuli and exercise adaptation such as muscle hypertrophy. However, the acute and long-term adaptational response of STARS is still unclear. This study aimed at investigating the acute and long-term endurance training response on the mRNA and protein expression of STARS and its related upstream and downstream factors in human skeletal muscle. Methods: mRNA and protein levels of STARS and related factors were assessed in skeletal muscle of healthy young men and women following an acute bout of endurance exercise (n=20) or 12 weeks of one-legged training (n=23). Muscle biopsies were obtained before (acute and long-term), at 30 min, 2h and 6h following acute exercise, and at 24 hrs following both acute exercise and long-term training. Results: Following acute exercise, STARS mRNA was significantly elevated 3.9-fold at 30 minutes returning back to baseline 24 hours after exercise. STARS protein levels were were numerically but non-significantly increased 7.2-fold at 24 hours. No changes of STARS or ERRα mRNA or STARS protein expression were seen following long-term training. PGC-1α mRNA increased 1.7-fold following long-term training. MRTF-A mRNA was increased both following acute exercise and long-term training, in contrast to SRF mRNA and protein which did not change. Conclusion: STARS mRNA is acutely upregulated with exercise, but there is no cumulative effect to long-term training as seen in PGC-1α mRNA expression. Exercise intensity might play a role in manifestation of protein expression, suggesting a more complex regulation of STARS.

研究目的：Rho信号通路条纹状激活蛋白（Striated Activator of Rho-Signaling, STARS）是连接外部刺激与肌肉肥大等运动适应过程的关键因子，但目前有关STARS的急性及长期适应性反应的研究仍不明确。本研究旨在探究单次及长期耐力训练对人骨骼肌中STARS及其上下游相关因子的mRNA与蛋白质表达的影响。
研究方法：本研究纳入20名健康青年男女，接受单次耐力运动刺激，分别于运动前、运动后30min、2h、6h及运动后24h采集骨骼肌活检样本（n=20）；另纳入23名受试者进行12周单腿耐力训练，分别于训练前、急性运动后24h及长期训练后24h采集样本（n=23）。检测STARS及相关因子的mRNA与蛋白质表达水平。
研究结果：单次运动后，STARS的mRNA表达在30min时显著升高3.9倍，至运动后24h恢复至基线水平；STARS蛋白质表达在24h时升高7.2倍，但未达到统计学显著性。长期训练后，STARS、ERRα的mRNA表达及STARS蛋白质表达均无显著变化；PGC-1α的mRNA表达升高1.7倍。MRTF-A的mRNA表达在单次运动及长期训练后均显著上调，而SRF的mRNA及蛋白质表达无明显改变。
研究结论：STARS的mRNA表达可随单次运动急性上调，但未如PGC-1α mRNA一般在长期训练中呈现累积效应。运动强度可能对STARS蛋白质表达的显现具有调控作用，提示STARS的表达调控机制更为复杂。