Altered gene expression in the small intestine of Mfge8 null and Cd36 null mice

We aimed to investigate the role of Mfge8 and Cd36 in intestinal lipid metabolism. We looked at the effect of the genetic deletion of Mfge8 and Cd36 in the small intestine by 3' Tag RNA sequencing. We obtained primary enterocytes from WT and Mfge8 null mice, isolated RNA and performed 3'Tag RNA sequencing. We also isolated small intestinal RNA from WT and Cd36 null mice and then performed 3'Tag RNA sequencing.