Nematode surface chemistry dataset

This dataset contains surface chemistry profiles of wild-type Caenorhabditis elegans (N2) and Pristionchus pacificus (PS312), along with their respective daf-22 mutant strains (C. elegans DR476 and P. pacificus RS2770/RS5204), each captured in biological replicates (n = 9). Data were collected using 3D-OrbiSIMS mass spectrometry with an argon gas cluster ion beam (Ar3000+, 20 keV) for surface sputtering and an Orbitrap analyser for high-resolution detection (mass resolving power >240,000 at m/z 200). Surface analysis was confined to the outermost ~50 nm, enabling detailed profiling of species-specific and developmentally regulated lipid compositions. This dataset supports research into nematode physiology, lipid metabolism, and interspecies interactions.

本数据集涵盖野生型秀丽隐杆线虫（Caenorhabditis elegans，N2）与太平洋拟杆线虫（Pristionchus pacificus，PS312）的表面化学特征谱，及其各自对应的daf-22突变株（秀丽隐杆线虫DR476、太平洋拟杆线虫RS2770/RS5204）；所有样本均设置生物学重复（biological replicates，n=9）。数据通过3D-OrbiSIMS质谱法采集，采用氩气团簇离子束（argon gas cluster ion beam，Ar3000+，20 keV）完成表面溅射，搭配Orbitrap分析器开展高分辨检测，其在m/z 200处的质量分辨率大于240,000。本次表面分析的范围限定于样品最外层约50 nm的区域，可实现物种特异性及发育调控型脂质组成的精细表征。本数据集可为线虫生理学、脂质代谢及种间互作相关研究提供有力支撑。