Coordination of Rel Expression by MAP3K4, CBP, and HDAC6 Controls TS cell Phenotype (RNA-seq)

Trophoblast stem cells lack MAP3K4 activity (TSKI4 cells) switch from epithelial phenotype to intermediate phenotype. Loss of epithelial phenotype is due to the loss of CBP histone acetyltransferase activity and the gain of histone deacetylase HDAC6 expression and activity. In our work, we identify a small network of 183 genes whose expression is co-regulated by MAP3K4, CBP, and HDAC6. Further, we define the key role of one of these co-regulated genes, Rel, in inducing epithelial phenotype in intermediate TSKI4 cells.

缺乏丝裂原活化蛋白激酶激酶激酶4（MAP3K4）活性的滋养层干细胞（TSKI4细胞）会从上皮表型转变为中间表型。上皮表型的丧失源于CREB结合蛋白（CBP）的组蛋白乙酰转移酶活性缺失，以及组蛋白去乙酰化酶HDAC6的表达与活性上调。本研究中，我们鉴定出一个由183个基因组成的小型调控网络，这些基因的表达受MAP3K4、CBP及HDAC6共同调控。此外，我们明确了上述共同调控基因之一——Rel——在中间表型TSKI4细胞中诱导上皮表型的关键作用。