SH1042542.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流及开展基于DNA的物种假说（Species Hypotheses，以下简称SH）相关工作。国际核酸序列数据库中的全部真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一组动态距离阈值（0.5%~3.0%），被聚类至近似物种层级。所有SH均被赋予以数字对象标识符（Digital Object Identifier，DOI）格式命名的唯一稳定标识，其分类学与生态学注释通过分布式网页端第三方注释流程完成校验。每个SH均会尽可能结合其包含的全部序列的鉴定结果，与对应分类单元名称及其分类层级（如门、纲、目等）建立关联。将选取一条自动或人工指定的序列作为各SH的代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科研界用于本地序列相似性检索、下一代测序（Next-Generation Sequencing）分析流程等研究场景。随着公共真菌ITS序列数据量的增长，该系统与配套数据集将自动完成更新。