Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

ABSTRACT In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.

摘要：本研究以农业工业废弃物剑麻汁为底物，采用静置培养方式，利用汉森糖杆菌（Komagataeibacter hansenii）ATCC 23769优化细菌纤维素（bacterial cellulose，BC）的合成工艺。本研究首先通过单因素变量法对发酵参数进行优化，考察了初始糖浓度、pH值、氮源补充量及培养时间对细菌纤维素产量的影响；随后采用中心复合旋转设计（central composite rotational design，CCRD）探究了氮源种类与添加量对细菌纤维素合成的调控作用。结果显示，当采用初始糖浓度为15 g/L、pH值为5的剑麻汁培养基，添加7.5 g/L酵母提取物，静置培养10天后，可获得最高细菌纤维素产量（3.38 g/L）。在该优化后的剑麻汁培养条件下，细菌纤维素的产率为合成培养基的3倍，证实剑麻汁是制备细菌纤维素的适宜底物。