Supplementary information of Novel therapeutic strategy for intractable keloids: Suppression of intracellular mechanotransduction and actin polymerization via Rho-kinase pathway inhibition

Supplementary materials and supplementary figure legends.
Supplementary figure S1. Expression of ROCK1 in keloid tissues and fibroblasts.
Supplementary figure S2. Mechanical stress induces ROCK1 expression and F-actin rearrangement in keloid fibroblasts.
Supplementary figure S3. Suppressed ROCK1 induces F-actin rearrangement in KFs through Rho/ROCK1 transduction pathway.
Supplementary figure S4. Effect of Y27632 on cytoskeletal rearrangement of F-actin in KFs.
Supplementary figure S5. Inhibition of ROCK1 attenuates skin fibrosis and cell migration in KFs.
Supplementary figure S6. Cyclic stretch-activated ROCK1 triggers nuclear translocation of YAP and MRTF.
Supplementary figure S7. Histological and immunohistochemical images of nodules formed by human KFs in SCID mice 7 days post-Y27632 treatment.
Supplementary figure S8. Histological images of nodules formed by human KFs in SCID mice 7 days post-Y27632 treatment.
Supplementary figure S9. Immunohistochemical images of the nodules formed by human KFs in SCID mice 7 days post Y27632 treatment.

补充材料及补充图例说明。
补充图S1. 疙痕组织中ROCK1的表达及成纤维细胞。
补充图S2. 机械应力诱导的ROCK1表达及F-actin重排于疙瘩成纤维细胞。
补充图S3. 抑制的ROCK1通过Rho/ROCK1转导途径诱导KFs中的F-actin重排。
补充图S4. Y27632对KFs中F-actin细胞骨架重排的影响。
补充图S5. ROCK1抑制减轻KFs中的皮肤纤维化和细胞迁移。
补充图S6. 循环拉伸激活的ROCK1触发YAP和MRTF的核转位。
补充图S7. Y27632治疗7天后，SCID小鼠中由人KFs形成的结节的组织学及免疫组织化学图像。
补充图S8. Y27632治疗7天后，SCID小鼠中由人KFs形成的结节的组织学图像。
补充图S9. Y27632治疗7天后，SCID小鼠中由人KFs形成的结节的免疫组织化学图像。