16S rRNA sequencing of the ileal and cecal microbiota in Wuhuang pigs and their crossbred offspring

This project characterizes the microbial community structure in the digestive tract of Wuhuang pig, a local Chinese breed, and its crossbred offspring. We collected content samples from the ileum and cecum of these pigs and performed 16S rRNA gene sequencing to analyze the composition and diversity of the gut microbiota. The aim is to understand how crossbreeding influences the gut microbiome in different intestinal locations, which may provide insights into pig health and productivity.