Diurnal patterns of gene expression in the dorsal vagal complex and the central nucleus of the amygdala

Micropunches of dorsal vagal complex (DVC) and central nucleus of the amygdala (CeA) were collected from male, Sprague Dawley rats at three different times of the day. Animals were not experimentally different in any way except for the time of sacrifice and collction. Gene expression of 145 genes were measured with Fluidigm's Biomark 96.96 platform.本研究从每日三个不同时间点的雄性斯普拉格·道利（Sprague Dawley）大鼠体内采集迷走神经背侧复合体（dorsal vagal complex, DVC）与杏仁核中央核（central nucleus of the amygdala, CeA）的微穿刺取材样本。除安乐死时间与样本收集环节外，所有实验动物未在任何实验层面存在差异。研究采用Fluidigm公司的Biomark 96.96平台对145个基因的表达水平进行了检测。