Additional file 7 of Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors

Supplementary file 1. Raw spike counting and bar charts of ac4-enhanced activation from various transgenic flies. Control fly line genotypes: Oregon-C wild type, w;IR75d-Gal4;IR75dKO, w;UAS-IR75dHEK;IR75dKOand w;Bl/CyO;IR84a-Gal4KI. Fly-line genotypes expressing heterologous IRs: w;IR76a-Gal4;UAS-CpomIR41a1, w;IR75d-Gal4/UAS-DsuzIR75dHEKK;IR75dKO, w;UAS-DsuzIR64a;IR84a-Gal4KI, and w;UAS-CpomIR64a∆;IR84a-Gal4KI. Choice of p-values from a parametric or a non-parametric statistical analysis depended on tests for normal distribution. The magnitude of p-values has been distinguished as it follows: * p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001; which is consistent with Figure 1. Note: colors have been used to distinguish genotypes and remark their significant effects based on colors used in Figure 1. For the tested ligands, blue names indicate ligands diluted in water and red ligands diluted in ethanol.

补充文件1：各类转基因果蝇经ac4增强激活后的原始尖峰计数与柱状图。对照果蝇品系基因型包括：野生型Oregon-C、w;IR75d-Gal4;IR75dKO、w;UAS-IR75dHEK;IR75dKO以及w;Bl/CyO;IR84a-Gal4KI。表达异源离子型受体（Ionotropic Receptor，IR）的果蝇品系基因型包括：w;IR76a-Gal4;UAS-CpomIR41a1、w;IR75d-Gal4/UAS-DsuzIR75dHEKK;IR75dKO、w;UAS-DsuzIR64a;IR84a-Gal4KI以及w;UAS-CpomIR64a∆;IR84a-Gal4KI。参数统计检验与非参数统计检验的p值选择需依据正态分布检验结果确定。p值的显著性分级标准如下：* 代表p<0.05；** 代表0.001<p<0.01；*** 代表p<0.001，该分级规则与图1保持一致。注：本文件采用与图1一致的配色方案，以区分不同果蝇品系基因型并标注其效应的显著性。对于受试配体，名称以蓝色标注者表示以水为溶剂稀释，红色标注者表示以乙醇为溶剂稀释。