Additional file 1 of Two lymphoid cell lines potently silence unintegrated HIV-1 DNAs

Additional file 1: Figure S1. Viral protein Vpr relieves silencing of unintegrated HIV-1 DNA. HeLa cells were infected with HIV-1 reporter virus expressing ZsGreen, either integration-competent (IN-wt, black curves) or integration-deficient (IN-D64A, red curves). A Vpr-positive reporter genome was generated by restoring the ORF sequence to the parent viral genome. Cells were scored by flow cytometry for % ZsGreen (upper panels) and Mean Fluorescence Intensity (MFI, lower panels). A Vpr-minus vector. B Vpr-positive vector.

附加文件1：图S1。病毒蛋白Vpr可缓解未整合HIV-1 DNA的基因沉默。将海拉（HeLa）细胞用表达ZsGreen的HIV-1报告病毒感染，该病毒分为具有整合活性的野生型整合酶（IN-wt，黑色曲线）与整合缺陷型（IN-D64A，红色曲线）两类毒株。通过将开放阅读框（Open Reading Frame, ORF）序列恢复至亲本病毒基因组，构建得到Vpr阳性报告病毒基因组。采用流式细胞术对细胞进行检测分析，统计ZsGreen阳性细胞占比（上图）与平均荧光强度（Mean Fluorescence Intensity, MFI，下图）。A：Vpr阴性载体；B：Vpr阳性载体。