Physico-chemical properties of scFv-C4 intrabodies.

Physico-chemical determinants of soluble intrabody expression. Solubility is improved by an overall negative charge at pH 7.4 and reduced GRAVY score. The GRAVY score is the average hydropathy score for all the amino acids in the protein, with a more negative score indicating increased hydrophilicity. The PEST-FIND program http://emboss.bioinformatics.nl/cgi-bin/emboss/pestfind objectively produces a score ranging from about −50 to +50. A score above +5 denotes a PEST region [33].