Systematic assessment of freely-diffusing single-molecule fluorescence detection using Brownian motion simulations - photon timetag simulation files
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Attached are the photon timestamp and channels simulated for different 3D diffusing molecules simulations at different conditions (simulation was performed by PyBroMo). Each of the files has, in its name, a code. The meaning of the codes are as following: f32445 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s 4b2894 - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s d28307 - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s 59d61c - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s e163e9 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s 85b0a1 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50



