Transcriptome-wide identification of IGF2BP binding RNAs by RIP-seq.
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https://www.ncbi.nlm.nih.gov/sra/SRP094048
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资源简介：
We identified the mRNA targets of the insulin-like growth factor-2 (IGF2) mRNA-binding proteins 1, 2, and 3 (IGF2BP1/2/3) by RNA immunoprecipitation and sequencing (RIP-seq). HEK293T cells transfected with Flag-tagged IGF2BP1/2/3 plasmids were expanded and UV-crosslinked before harvest. We performed RIP of individual IGF2BP using anti-Flag antibody from nuclear extractions, and identified the associated mRNAs by next generation sequencing. More than 5000 transcripts, including protein coding and non-coding transcripts, were identified from each RIP-seq sample. Overall design: RIP-seq in HEK293T cells with Flag-tagged IGF2BP1/2/3 overexpression.
创建时间：
2023-01-11
