Dataset related to article: "ChemCell: Bioorthogonal surface reprogramming of natural killer cells for antibody-directed cancer immunotherapy".

This dataset contains raw flow cytometry (.fcs) data and associated metadata supporting the study "ChemCell: Bioorthogonal surface reprogramming of natural killer cells for antibody-directed cancer immunotherapy". The dataset includes experiments analyzing surface modification of cells, antibody binding, antigen density, cytotoxic activity of NK cells, and effector–target cell interactions. Flow cytometry data were acquired using multiple instruments (including BD FACSymphony, LSRFortessa, CytoFLEX LX, and Aurora) and analyzed using FlowJo software (version 10, BD Biosciences). Data are organized into folders corresponding to figures in the manuscript, with file names reflecting experimental conditions (e.g., control, ChemCell-modified, time point, donor). A detailed description of experiments and file organization is provided in the accompanying README file. Representative gating strategies are available in the Supplementary Figures of the associated manuscript, and full analysis workflows are provided via FlowJo workspace files included in the repository. This dataset enables independent verification and reanalysis of the results presented in the study.