The whole transcriptome RNA sequencing of tumor tissue samples from 61 pancreatic ductal adenocarcinoma patients.

Tissue samples were collected from 61 stage I/II PDAC cases then were placed into 10% neutral buffered formalin solution, fixed at roomtemperature (8–72 h) and processed routinely into a paraffin embedded tissue block (FFPE). The RNAstorm™ Kit (biotium, USA) was used to extracted and isolated total RNA fraction from FFPE Tissue Samples. RNA libraries were prepared for sequencing using prepared SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian Kit. RNA-seq was performed by the Illumina sequencing platform on a 150-bp paired-end run. Overall design: The RNA expression profiles of formalin-fixed, paraffin-embedded PDAC tissue samples by whole transcriptome sequencing.