Investigation of the <i>Demodex</i> Lid Infestation with in Vivo Confocal Microscopy versus Light Microscopy in Patients with Seborrheic Blepharitis

The comparison of the diagnostic efficiency of a reference method (light microscopy [LM] using 10% potassium hydroxide [KOH]) with the use of 100% alcohol to <i>in vivo</i> confocal microscopy (IVCM) for the detection of <i>Demodex</i> eyelid infestation in seborrheic blepharitis patients. Eyelashes were epilated from the right eyes for the reference method and the left eyes for the alcohol group in 58 eyes of 29 patients. IVCM was used on the left eyes. The primary outcomes were the number of <i>Demodex</i> mites per lash and the rate of <i>Demodex</i> positivity (≥1 mite). The rate of <i>Demodex</i> positivity was similar among the three groups (KOH: 82.8%, alcohol: 86.2%, IVCM: 72.4%; <i>p</i> &gt;.05). The mean number of mites per lash in the KOH group (1.5 ± 1.3) was higher than in the alcohol (0.9 ± 0.6, <i>p</i> =.041) and IVCM groups (0.9 ± 0.9, <i>p</i> =.036). KOH was found to be superior in terms of the quantification of mites compared to alcohol and IVCM.本数据集旨在对比三种检测方法对脂溢性睑缘炎患者眼睑蠕形螨（Demodex）感染的诊断效能：采用10%氢氧化钾（KOH）的光学显微镜（LM）法、100%酒精处理法，以及活体细胞共聚焦显微镜（IVCM）法。本研究纳入29例患者共58只眼，其中参照方法（KOH法）采集患者右眼睫毛进行检测，酒精组采集左眼睫毛，IVCM法则对左眼进行检测。本研究的主要结局指标为每根睫毛上的蠕形螨数量，以及蠕形螨检出率（即检出≥1只螨的比例）。三组的蠕形螨检出率无显著统计学差异（KOH组：82.8%，酒精组：86.2%，IVCM组：72.4%；p>.05）。KOH组每根睫毛的平均螨数（1.5±1.3）高于酒精组（0.9±0.6，p=0.041）与IVCM组（0.9±0.9，p=0.036）。相较于酒精组与IVCM组，KOH法在螨体定量检测方面表现更优。