Insight into late synovial joint development by single cell transcriptomics in murine

We performed single cell RNA-Seq analysis of 2,267 cells from E17.5 murine knee joint. Using computational analytics and in situ hybridization, we identified five sub-populations contributing to joint constituents, and the underlying transcriptional networks, signaling, and core regulators involving in potential lineage transition during late joint developmental stage. We sampled Gdf5-lineage cells of the murine knee at embryonic time points e17.5 and subjected it to single cell RNAseq analysis via 10x.本研究对来自胚胎发育第17.5天（E17.5）小鼠膝关节的2267个细胞开展了单细胞RNA测序（single cell RNA-Seq）分析。结合计算分析与原位杂交（in situ hybridization）技术，本研究鉴定出5个构成关节组织的细胞亚群，并解析了关节发育晚期潜在谱系转换过程中所依赖的核心转录调控网络、信号通路及核心调控因子。本研究在胚胎发育第17.5天采集了小鼠膝关节的Gdf5谱系细胞，并通过10x技术对其开展了单细胞RNA测序分析。