High level, constitutive expression of DsRed or Tomato causes minimal disruption to virulence.
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a: Challenge by intranasal instillation; mice pre-treated with 500 µg Fe+2 just prior to challenge; dose is equivalent to 50X LD50 for wild type KIM D27.b: Challenge by subcutaneous injection; dose is equivalent to 50X LD50 for wild type CO92.
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2015-12-02



