Genome-wide analysis of Schwann cell gene expression in LKB1-SCKO mutant mice prior to occurrence of axon degeneration

Analysis of gene expression in LKB1(Stk11)-depleted sciatic nerves (LKB1-SCKO) vs control nerves from age-matched mice. Gene expression profiles are predominantly derived from Schwann cell glia and provide important information about the response of peripheral nerve Schwann cells to inactivation of the metabolic regulator protein LKB1(aka Stk11). Total RNA obtained from sciatic nerve segments from 6 LKB1-SCKO mutant mice compared to RNA from nerve segments from 6 control mice (floxed LKB1 mutant mice that do not express Cre recombinase).本数据集针对年龄匹配小鼠的LKB1（Stk11）敲除坐骨神经（LKB1-SCKO）与对照坐骨神经开展基因表达分析。该基因表达谱主要源自施万细胞（Schwann cell）胶质，可为解析外周神经施万细胞对代谢调节蛋白LKB1（又称Stk11）失活的应答机制提供关键实验依据。本研究从6只LKB1-SCKO突变小鼠的坐骨神经节段中提取总RNA，并与6只对照小鼠（未表达Cre重组酶（Cre recombinase）的floxed LKB1突变小鼠）的坐骨神经节段RNA进行对比分析。