Data from: Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

There is a need to develop economical, efficient and widely available therapeutic approaches to enhance the rate of skin wound healing. The optimal outcome of wound healing is restoration to the pre-wound quality of health. In this study we investigate the cellular response to biological stimuli using functionalized nanofibers from the self-assembling peptide, RADA16. We demonstrate that adding different functional motifs to the RADA16 base peptide can influence the rate of proliferation and migration of keratinocytes and dermal fibroblasts. Relative to unmodified RADA16; the Collagen I motif significantly promotes cell migration, and reduces proliferation.

当前亟需开发经济高效、易于获取的治疗手段，以加快皮肤创面的愈合速率。创面愈合的最优结局是使皮肤恢复至受伤前的健康状态。本研究采用自组装肽（self-assembling peptide）RADA16制备的功能化纳米纤维（functionalized nanofibers），探究细胞对生物刺激的应答反应。本研究证实，在RADA16基础肽段上添加不同功能基序（functional motifs），可对角质形成细胞（keratinocytes）与皮肤成纤维细胞（dermal fibroblasts）的增殖及迁移速率产生调控作用。相较于未修饰的RADA16，胶原蛋白I（Collagen I）基序可显著促进细胞迁移并抑制细胞增殖。