Bacterial production in water samples collected at Station MARCH-1997-GN36199704601MSB01 in the Aegean Sea

Bacterial production was estimated by the 3H-leucine approach (Kirchman, Newell, Hodson, 1986). For each depth duplicate samples and a control were incubated with 1 nM L-[4,5 3H]-leucine (specific activity 128 Ci/mmol in March, 158 Ci/mmol in September) +18 nM non radioactive leucine. Samples were incubated in the dark at in situ temperature. Bacterial production was calculated according to Kirchman (1993) from 3H-leucine incorporation rates.

细菌生产力采用氚标记亮氨酸（3H-leucine）法进行估算（Kirchman、Newell、Hodson，1986）。各深度层均设置平行样品与对照样品，均加入1 nM的L-[4,5 3H]-亮氨酸（比活度：3月为128 Ci/mmol，9月为158 Ci/mmol）与18 nM非放射性亮氨酸进行孵育。样品于原位温度下的黑暗环境中孵育。细菌生产力依据Kirchman（1993）提出的方法，通过3H-亮氨酸掺入速率计算得到。