Differential expression genes after powdery mildew infection in wheat

Transcriptome sequencing of the wheat seedling RNA samples gave 63.26 million (M) reads for J0, 62.46 M for J12, 55.88 M for N0 and 62.67 M reads for N12. The percentage of reads with Phred quality scores (Q-scores) > Q20 was >96%, which indicated that there were <1% errors (Table 1). Sequencing reads were then mapped to the wheat genome (Ensembl Genomes release-35). Approximately 87% of reads from each library were mapped perfectly to the reference genome, and the average value of uniquely mapped reads was ~79%.