Establishing reference values of amino acids and acylcarnitines from 16 species of stingless bees (Hymenoptera: Meliponini) from Mexico

Four supplementary Excel files are provided to support the data and statistical analysis presented in this study: RawData_AA.xlsx – Contains the raw concentration values (in μmol/L) for the 12 amino acids measured in triplicate for each of the 16 stingless bee species analyzed. RawData_AC.xlsx – Contains the raw concentration values (in μmol/L) for the 28 acylcarnitines measured in triplicate for each of the 16 stingless bee species analyzed. Results_AA.xlsx – Provides the full statistical analysis for amino acids: Sheet 1 (Basic_Stats): Mean and standard deviation of each metabolite per species. Sheet 2 (Kruskal_Wallis): Non-parametric comparison across species. Sheet 3 (Dunn_Test): Post hoc pairwise comparisons. Sheet 4 (Bonferroni): Adjusted p-values for multiple comparisons. Sheet 5 (Significant): Metabolites with statistically significant differences (Bonferroni-corrected p < 0.05). Sheet 6 (Composition): Relative abundance (%) of each amino acid per species. Results_AC.xlsx – Provides the full statistical analysis for acylcarnitines: Sheet 1 (Basic_Stats): Mean and standard deviation of each metabolite per species. Sheet 2 (Kruskal_Wallis): Non-parametric comparison across species. Sheet 3 (Dunn_Test): Post hoc pairwise comparisons. Sheet 4 (Bonferroni): Adjusted p-values for multiple comparisons. Sheet 5 (Significant): Metabolites with statistically significant differences (Bonferroni-corrected p < 0.05). Sheet 6 (Composition): Relative abundance (%) of each acylcarnitine per species. AA_PLSDA.xlsx — PLS-DA outputs for amino acids: Sheet 1 (Scores): Sample-level PLS-DA scores for components (Comp1, Comp2, …) and sample metadata (sample ID, species, abbreviated species label). Use these coordinates for score plots. Sheet 2 (VIP): Variable Importance in Projection (VIP) values for each metabolite across components; includes component-specific VIPs and mean/summary VIPs used to rank discriminant metabolites. AC_PLSDA.xlsx — PLS-DA outputs for acylcarnitines (same structure as AA_PLSDA_results.xlsx): Sheet 1 (Scores): Sample-level PLS-DA scores (Comp1, Comp2, …) and sample metadata. Sheet 2 (VIP): Component-wise VIP values and mean VIPs for each acylcarnitine.