Transcriptomic analysis of C. elegans mutants which do not respond to oomycete exposure

We have established epidermal expression of chil-27p::GFP as the marker for activation of oomycete recognition response (ORR) in C. elegans. Through a forward genetic screen, we identified clec-27, clec-35, and ceh-37 to be the key players required for activating chil-27p::GFP upon exposure to the oomycete Myzocytiopsis humicola extract. To determine if activation of entire ORR is affected in these mutants, we analyzed their transcriptome in the presence and absence of extract and compared with that of wildtype C. elegans. Overall design: Comparative gene expression of M. humicola extract treated N2, ceh-37(ok642), clec-27(icb104), and clec-35(icb105) animals at L4 stage with untreated controls.