CIL:21784

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with mad2 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc).

本数据集包含5组经DNA染料Hoechst 33342染色的黑腹果蝇（Drosophila melanogaster）Kc167细胞样本。其中最后一组样本标记为nodsRNA，编号CIL 21789，为野生型细胞样本；其余4组样本分别标记为48、340、Anillin与mad2，对应编号依次为CIL 21749、21739、21759及21773，每组均通过RNA干扰（RNAi）技术敲低了不同的靶基因，其中标记为mad2的样本组为经mad2 RNAi处理的实验组。每组样本包含10张图像，总计50张。所有图像均通过Zeiss Axiovert 200M显微镜采集。该数据集的推荐应用场景为通过算法计算单张图像中的细胞数量。包含该细胞计数信息的制表符分隔文本文件可在标注框中给出的URL处获取。使用本图像集时的推荐引用格式为：本研究使用了来自Broad生物图像基准集（Broad Bioimage Benchmark Collection，www.broad.mit.edu/bbbc）的黑腹果蝇Kc167细胞图像集（Carpenter等，《基因组生物学》，2006年）。