Measures of cross-pollination and yield production of faba bean (Vicia faba L.) in response to heat stress and insect pollination treatments

Datasets relating to two experiments measuring cross-pollination and yield production of faba bean (Vicia faba L, cultivar Wizard) in response to heat stress and insect pollination treatments. This accompanies the paper titled 'Elevated temperature drives a shift from selfing to outcrossing in the insect pollinated legume, faba bean (Vicia faba).' Journal of Experimental Botany. Bishop J, Jones HE, O'Sullivan DM, Potts SG. (In press). Potted faba bean plants were exposed to either heat stress (30/24°C day/night temperature, 16 hour photoperiod) or control (20/14°C) treatments for five days during floral development and anthesis. These plants were then either i) moved to mesh cages that either contained bumblebees or were empty, or ii) moved to two field sites and either left open to allow visitation by wild insect pollinators, or enclosed individually in mesh bags. Cross-pollination was assessed using a morphological marker. Both the mesh cages and field contained pollen donor plants that were homozygous for a dominant 'dark hilum' trait. The experimental plants were homozygous for a recessive 'light hilum' trait. Thus, by observing which colour hila the experimental progeny produced, it was possible to identify individuals that had been sired by the pollen donor plants. Yield production parameters were assessed on the experimental plants at senescence. The effects of individual mesh bags used to exclude insect pollinators in the field experiment were established using two separate experiments. Separate groups of plants were either i) all bagged at the field sites, with a subset of flowers hand pollinated to establish the effect of bagging on yield production per flower while maximizing pollination, or ii) housed within a large mesh cage to exclude all insect pollinators, then either bagged or unbagged at the same time as the field experiment was conducted, with whole plant yield parameters then measured at senescence.

本数据集围绕两项针对蚕豆（Vicia faba L.，品种Wizard）的实验构建，旨在探究热胁迫与昆虫授粉处理对其异交授粉与结实产量的影响。本数据集配套发表于《实验植物学杂志》（*Journal of Experimental Botany*）的论文《升温驱动虫媒豆科作物蚕豆（Vicia faba）从自交向异交转变》，作者为Bishop J、Jones HE、O'Sullivan DM、Potts SG，文章即将刊出。盆栽蚕豆植株在花发育与开花期，分别接受为期5天的热胁迫（heat stress）处理（昼/夜温度30/24℃，光周期16小时）或对照处理（20/14℃）。处理完成后，植株被分为两组：①转移至分别放置熊蜂（bumblebees）或无蜂的网笼（mesh cage）中；②转移至两处田间样地，要么保持开放以允许野生昆虫授粉者（wild insect pollinators）访花，要么单独套入网袋。异交授粉通过形态标记进行评估：网笼与田间样地均设置了携带显性‘深色种脐’（dominant 'dark hilum'）性状纯合基因型的花粉供体植株，实验植株则携带隐性‘浅色种脐’（recessive 'light hilum'）性状纯合基因型。因此，通过观察实验子代的种脐颜色，即可鉴定出由花粉供体植株授粉产生的子代个体。在植株衰老期，对实验植株的产量相关参数进行测定。田间实验中用于排除昆虫授粉者的单独网袋效应，通过两项独立实验进行验证。另有两组独立的植株分别接受如下处理：①在田间样地全部套袋，并对部分花朵进行人工授粉，以在最大化授粉的前提下评估套袋对单花结实的影响；②置于大型网笼中以排除所有昆虫授粉者，在田间实验开展同期进行套袋或不套袋处理，待植株衰老后测定整株产量参数。