Data undelying the research on: New culture medium for micropropagation protocol of guava (Psidium guajava) seedlings, a plant with interest in cool subtropics.docx

<i>Psidium guajava is a new crop cultivated in Greece, with a potential pharmaceutical and ornamental uses. An efficient and reliable protocol for in vitro clonal propagation of P. guajava from seeds was developed. Five different media were examined. Shoot proliferation succeeded on a new basal medium (BB) with extra vitamin solution, without plant growth regulators. In addition, shoots on media supplemented with BB vitamins solution performed better than in MS and WP media. When BB medium was used, the greatest shoot number resulted in 4 mg L-1 BA with a score of 2.8. Regenerated single shoots were rooted in the BB medium, supplemented with 0.1 ΝΑΑ (mg L-1) and producing 2.2 roots of 4.6 cm, along with 3.1 cm of adventitious shoots. Acclimatization was accomplished in three phases, using peat-moss, vermiculite and perlite (1:1:1), declining a total survival of 83.3%. In order to examine the genetic diversity of the plant material, ISSR and SCoT molecular markers were used. The results indicated a low diversity, along with high percentage of polymorphism for both ISSR and SCoT analyzes.</i><br>

番石榴（Psidium guajava）是希腊新近栽培的经济作物，兼具潜在药用与观赏价值。本研究建立了一套高效稳定的种子来源番石榴体外无性繁殖体系。试验共评估了5种不同培养基：在添加额外维生素溶液且不含植物生长调节剂的新型基础培养基（BB）上，可成功实现芽增殖；此外，添加BB维生素溶液的培养基，其芽生长表现优于MS（Murashige and Skoog）培养基与WP培养基。当使用BB培养基时，添加4 mg·L⁻¹ 6-苄氨基腺嘌呤（BA）可获得最多芽数，增殖评分为2.8。再生单芽在添加0.1 mg·L⁻¹ α-萘乙酸（NAA）的BB培养基中完成生根，每芽可生成2.2条长度为4.6 cm的不定根，同时诱导出长度为3.1 cm的不定芽。驯化过程分为三个阶段，采用泥炭藓、蛭石与珍珠岩按1:1:1的体积比混合作为基质，最终总成活率达83.3%。为探究该植物材料的遗传多样性，本研究采用ISSR（简单序列重复间区）与SCoT（目标起始密码子多态性）两种分子标记技术进行分析。结果显示，ISSR与SCoT检测均呈现较低的遗传多样性，但二者的多态性百分比均较高。