SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in July 2014

Flow cytometry data was collected in July 2014, in waters off South Australia. \n\n\nThe general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.\n\nSix main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

流式细胞术数据采集于2014年7月，采集区域为澳大利亚南部近海海域。

本研究的核心目标为建立澳大利亚南部大陆架海域生态系统的背景知识体系，阐明上升流/咸水外泄事件对微生物群落的影响，最终构建该区域的生物地球化学模型。样本采集依托澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在RV Ngerin号科考船上开展的航次中完成。各航次均针对深度叶绿素最大值层（Deep Chlorophyll Maximum, DCM）的物理、化学与生物学特性开展调查，并针对微微型浮游植物、细菌与病毒采集流式细胞术数据。

本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、B4（35.168°S，135.418°E）与B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需注意的是，结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）与科考船平均航速（9节）可判定，各站位采集的水团互不相同。研究期间还偶尔从袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S，136.447°E）以及发生咸水外泄的斯宾塞湾口锚定站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集补充样本。