Knockdown of SNHG1 inhibits cervical cancer growth through sponging miR-194 to regulate HCCR

To investigate the mechanism of small nucleolar RNA host gene 1 (SNHG1) in cervical cancer (CC). <b>Methods:</b> The expression of SNHG1, miR-194 and human cervical cancer oncogene (HCCR) in CC tissues and cells was detected using qRT-PCR and western blot. The interaction among the three molecules was measured using dual-luciferase reporter assay and RNA immunoprecipitation assay. The function of SNHG1 in CC cells was detected by CKK-8 assay and flow cytometry analysis. <b>Results:</b> SNHG1 was highly expressed in CC tissues and CC cell lines. Knockdown of SNHG1 inhibited CC cell proliferation and enhanced the ability of cell apoptosis. Mechanism investigation revealed that SNHG1 modulated HCCR expression <i>via</i> acting as a competing endogenous RNA of miR-194. Moreover, miR-194 inhibitor changed the effects of si-SNHG1 on CC cells growth. <i>In vivo</i> experiment, silencing of SNHG1 suppressed CC tumor growth by modulating miR-194/HCCR axis. <b>Conclusion:</b> Knockdown of SNHG1 inhibited CC progression by targeting HCCR <i>via</i> sponging with miR-194.

为探讨小核仁RNA宿主基因1（small nucleolar RNA host gene 1, SNHG1）在宫颈癌（cervical cancer, CC）中的作用机制。**方法：** 采用实时定量逆转录聚合酶链反应（quantitative real-time polymerase chain reaction, qRT-PCR）和蛋白质印迹法（western blot）检测宫颈癌组织及细胞中SNHG1、miR-194与人宫颈癌癌基因（human cervical cancer oncogene, HCCR）的表达水平；采用双荧光素酶报告基因实验（dual-luciferase reporter assay）与RNA免疫沉淀实验（RNA immunoprecipitation assay）检测三者间的相互作用；采用CKK-8实验及流式细胞术分析检测SNHG1在宫颈癌细胞中的功能。**结果：** SNHG1在宫颈癌组织及宫颈癌细胞系中呈高表达。沉默SNHG1可抑制宫颈癌细胞增殖并促进细胞凋亡。机制研究显示，SNHG1可通过作为miR-194的竞争性内源RNA（competing endogenous RNA, ceRNA）调控HCCR的表达。此外，miR-194抑制剂可逆转si-SNHG1对宫颈癌细胞生长的影响。*体内*实验表明，沉默SNHG1可通过调控miR-194/HCCR轴抑制宫颈癌肿瘤生长。**结论：** 沉默SNHG1可通过海绵吸附miR-194靶向调控HCCR，从而抑制宫颈癌的进展。