Sequence differences between strain BTV8/2007 and BTV8/Neth2006.

aNucleotide position numbering from strain BTV8/Neth2007 (genbank accession numbers GQ506451–GQ506460).bStrain BTV8/Neth2006/04 [5] has been isolated on KC cells and passaged one time on BHK21 cells before analysis. The sequences from the genome segments correspond to genbank accession numbers AM498051–AM498060 for Seg-1 to Seg-10.cStrain BTV8/Neth2006 [25] is the same strain as BTV8/Neth2006/04 but has been passaged 2 times extra on BHK21 cells. The sequences from the genome segments correspond to genbank accession numbers FJ183374–FJ183383 for Seg-1 to Seg-10.dBTV8/net07/e1/bhk3 (described in this paper) was isolated on eggs and was further passaged 3 times on BHK21 cells before analysis. This sequence matches completely to sequences determined by Maan et al (2010). See also footnote a.eAmino acid position numbering from strain BTV8/Neth2007.