Mobile And Real-time PLant disEase (MARPLE) diagnostics for strain-level identification of wheat yellow rust

We developed a portable, genomics-based, point-of-care approach specifically tailored for identifying individual strains of complex fungal plant pathogens. Using targeted sequencing, we overcame limitations associated with the sheer size of fungal genomes and their frequent obligately biotrophic nature. This new method called Mobile And Real-time PLant disEase (MARPLE) diagnostics enables rapid identification of both newly emergent strains and those with specific properties such as fungicide resistance.