Drought stress and high heat tolerance in domesticated Phaseolus beans

Abiotic constraints, such as drought and heat driven by climate change, negatively impact the production of the common bean (Phaseolus vulgaris L.), an essential grain legume worldwide. The ability to tolerate drought and heat stress in common bean can be improved by introducing genetic variation from related species, such as tepary bean (Phaseolus acutifolius A. Gray), which has recently gained attention because of its adaptation to drought and heat stresses and potential use as a genetic resource and alternative crop. To better understand the phenotypic response of tepary bean to drought and heat stress in multiple environments and trials and to select highly adapted tepary beans, we conducted two field experiments. In Experiment 1, we compared the adaptation to drought stress of tepary bean (n = 10), common bean (n = 10), and Lima bean (Phaseolus lunatus L.; n = 9) by assessing the reduction in grain yield under terminal drought compared to well-irrigated conditions in two California..., Experiment 1: Drought evaluation of three domesticated Phaseolus species
To compare the drought stress response among key domesticated Phaseolus species, ten genotypes of each of the common bean and tepary bean and nine genotypes of the Lima bean (Supplemental Table S1) were evaluated under well-irrigated and terminal drought conditions during the summer of 2016 in two locations in California (Table 1). These 29 genotypes are representative of the genetic diversity in these three species as they include representatives of the two domestications in common and Lima beans (e.g., Kwak and Gepts, 2009; Garcia et al., 2021) and their breeding status (landraces vs. improved varieties). Field trials were conducted at the University of California, Agricultural and Natural Resources West Side Research and Extension Center (WSREC) (Bsk climate, i.e. arid, cold steppe type climate, according to the Köppen-Geiger classification: Peel et al. 2007; Rubel & Kottek, 2010; Beck et al., 2018), and the..., , # Drought stress and high heat tolerance in domesticated Phaseolus beans

[https://doi.org/10.5061/dryad.zpc866tgq](https://doi.org/10.5061/dryad.zpc866tgq)

## Description of the data and file structure

### Files and variables

#### File: Experiment\_1\_Legend.docx

**Description:** Legend/abbreviations for Experiment_1.xlsx

#### File: Experiment\_2\_Legend.docx

**Description:** Legend/abbreviations for Experiment_1.xlsx

#### File: Experiment\_1.xlsx

**Description:** Comparison of drought stress tolerance among and within three domesticated* Phaseolus* (bean) species: common bean (*P. vulgaris*; n = 10), Lima bean (*P. lunatus*; n = 9), and tepary bean (*P. acutifolius*; n = 10). These were planted in 2016 in two locations with contrasting climate in the Central Valley of California: UC Davis and the West Side Research & Extension Center of UC ANR.

##### Variables

* Input variables: Species (3), locations (2), treatments (2), and genotypes (9 or 10; 29); Output variables: DF...

气候变化引发的干旱与高温等非生物胁迫，对全球重要豆科粮食作物普通菜豆（*Phaseolus vulgaris* L.）的生产造成负面影响。通过引入近缘物种的遗传变异，可提升普通菜豆的耐旱与耐热能力，其中尖叶菜豆（*Phaseolus acutifolius* A. Gray）因本身对干旱、高温胁迫的适应性，以及作为遗传资源与替代作物的应用潜力，近年来受到广泛关注。为明确尖叶菜豆在多环境与多试验下对干旱、高温胁迫的表型响应，并筛选出适应性优异的尖叶菜豆材料，本研究开展了两项田间试验。在试验1中，本研究通过对比加州两地灌溉充足与终期干旱胁迫下的籽粒产量降幅，评估了尖叶菜豆（n=10）、普通菜豆（n=10）以及利马菜豆（*Phaseolus lunatus* L.；n=9）的耐旱适应性。【试验1：3个驯化菜豆属物种的耐旱性评价】

为比较核心驯化菜豆属物种的干旱胁迫响应差异，本研究于2016年夏季在加州的两个试验点（表1），设置全灌溉与终期干旱两种处理，对普通菜豆、尖叶菜豆各10份基因型，以及利马菜豆9份基因型（补充表S1）进行了耐旱性评价。这29份基因型涵盖了三个物种的遗传多样性：普通菜豆与利马菜豆均包含两个驯化类群的代表性材料（如Kwak和Gepts，2009；Garcia等，2021），同时覆盖了地方品种与改良品种两种育种状态。田间试验分别设于加州大学农业与自然资源部西侧研究与推广中心（WSREC），该区域属于柯本-盖格气候分类法下的Bsk气候类型，即干旱寒冷草原气候（Peel等，2007；Rubel & Kottek，2010；Beck等，2018），以及……
# 驯化菜豆属物种的干旱与高温胁迫耐受性

[https://doi.org/10.5061/dryad.zpc866tgq](https://doi.org/10.5061/dryad.zpc866tgq)

## 数据与文件结构说明

### 文件与变量

#### 文件：Experiment_1_Legend.docx
**说明：** Experiment_1.xlsx 的图例与缩写说明

#### 文件：Experiment_2_Legend.docx
**说明：** Experiment_1.xlsx 的图例与缩写说明

#### 文件：Experiment_1.xlsx
**说明：** 本文件对比了3个驯化菜豆属（菜豆）物种间及种内的干旱胁迫耐受性：普通菜豆（*P. vulgaris*；n=10）、利马菜豆（*P. lunatus*；n=9）与尖叶菜豆（*P. acutifolius*；n=10）。所有材料于2016年种植于加州中央谷地气候差异显著的两个试验点：加州大学戴维斯分校，以及加州大学农业与自然资源部西侧研究与推广中心。

##### 变量
* 输入变量：物种（3类）、试验点（2个）、处理（2种）与基因型（9或10份，共29份）；输出变量：DF……