SH1042298.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）提供了统一的操作框架。国际核酸序列数据库中的所有真菌内部转录间隔区（Internal Transcribed Spacer，简称ITS）序列，均通过应用一系列取值范围为低于0.5%至3.0%的动态距离阈值，被聚类至近似物种水平的聚类单元。所有物种假说均会被分配一个以数字对象唯一标识符（Digital Object Identifier，简称DOI）为格式的唯一且稳定的专属名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作进行验证。研究人员会结合该物种假说下所有序列的鉴定结果，尽可能将其关联至对应的分类单元名称及其分类层级（如门、纲、目等）。针对每个物种假说，研究人员会选取一条经自动或人工指定的序列作为其代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科研社群用于本地序列相似性搜索、下一代测序分析流程等科研场景。随着公共真菌ITS序列的数量不断增长，该系统及其配套数据将自动完成更新。