Systematic identification of aberrant non-coding RNAs and their mediated modules in rotator cuff tears [miRNA-seq]

The purpose of this study is to identify dysregulated ncRNAs and understand how they influence Rotator cuff tears (RCT). We performed RNA sequencing and miRNA sequencing on 5 pairs of torn supraspinatus muscles and matched unharmed subscapularis muscles to identify RNAs dysregulated in RCT patients. Based on the results of differential expression analysis and miRNA targeting information, we constructed lncRNA/circRNA-associated dysregulated ceRNA networks in RCT. We found the largest module in the ceRNA network and identified several important ncRNAs in this module, which may have critical roles in RCT. 5 female patients with unilateral shoulder pain were enrolled in this study. The torn supraspinatus and intact subscapularis were confirmed by irrigation and debridement in intraarticular and subacromial space, then a small piece of muscle belly from each muscle was carefully collected by a grasper. After that, the supraspinatus tear was repaired using suture anchors. A total of 10 samples from 5 patients were stored in liquid nitrogen for further analysis. ***Raw data not available due to patient privacy concerns***