Pairwise multiplex CRISPR/Cas9 screen of mitophagy receptors in acute myeloid leukemia

The selective autophagic degradation of mitochondria via mitophagy is essential for preserving mitochondrial homeostasis and thereby disease maintenance and progression in acute myeloid leukemia. The process of mitophagy is orchestrated by a variety of mitophagy receptors whose interplay in AML is not well understood. Here, we established a dual multiplexed CRISPR screen targeting mitophagy receptors to elucidate redundancies and individual contributions of mitophagy receptors and gain a deeper understanding of the functional interactome governing mitophagy in AML. Pairwise multiplex CRISPR/Cas9 screen of mitophagy receptors for proliferation and DFP- or OA-induced mitophagy flux in acute myeloid leukemia cell line MV4-11