Symbiodinium diversity on the Great Barrier Reef (MTSRF Project 2.5i.3)

Samples of S. flexibilis were collected from a total of 11 sites from inshore and offshore reefs within the Torres Strait, Cairns, Townsville and Whitsunday regions of the Great Barrier Reef between December 1998 and February 2000. The microsatellite alleles of Symbiodinium C (loci SymC_3-01, SymC_3-02,SymC_3-03 and SymC_3-04) were analysed for their genetic diversity using the variables inshore/offshore reefs; molecular variance pairwise estimates (FST) of genetic differentiation; and hierarchical cluster analysis of Euclidean distances of frequencies grouped using the UPGMA (Unweighted Pair Group with Arithmetic mean) algorithm.
Site locations:Torres Straits (Dungeness); Cairns (Agincourt, Escape, Pickersgill and Undine Reefs); Townsville (Great Palm and Orpheus Islands, Rib and Trunk Reefs); Whitsunday (Hook and Deloraine Islands).

1998年12月至2000年2月，研究人员从大堡礁（Great Barrier Reef）范围内托雷斯海峡、凯恩斯、汤斯维尔及圣灵群岛区域的近岸与离岸礁礁区的共计11个采样点，采集了S. flexibilis样本。本研究针对虫黄藻C系（Symbiodinium C）的微卫星等位基因（位点包括SymC_3-01、SymC_3-02、SymC_3-03及SymC_3-04）开展遗传多样性分析，分析维度包含近岸/离岸礁生境变量、遗传分化的分子方差成对估计值（FST），以及基于等位基因频率欧氏距离的分层聚类分析——其中聚类分组采用UPGMA（非加权配对算术平均法，Unweighted Pair Group with Arithmetic Mean）算法。
采样点分布如下：
托雷斯海峡（邓杰内斯礁）；
凯恩斯区域（阿金库尔礁、埃斯开普礁、皮克瑟吉尔礁与厄丁礁）；
汤斯维尔区域（大棕榈岛、奥费斯岛、里布礁与特伦克礁）；
圣灵群岛区域（胡克岛与德洛莱恩岛）。