SH1042088.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播并处理基于DNA的物种假说（Species Hypotheses，简称SH）。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一系列动态距离阈值（0.5% 至 3.0%，阈值下限可小于0.5%）被聚类至近似物种水平的单元。所有物种假说均会被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，DOI）作为标识名称，其分类学与生态学注释信息将通过分布式的网络第三方注释工作完成验证。SH将尽可能结合该假说下所有序列的鉴定结果，关联至对应的分类单元名称及其分类层级（如门、纲、目等）。将为每个此类物种假说选定一条经自动或人工指定的序列作为其代表序列。这些代表序列已通过官方存储库链接https://unite.ut.ee/repository.php 向科学界公开发布，可用于本地序列相似性检索、高通量测序（Next-Generation Sequencing）分析流程等科研场景。随着公共真菌ITS序列数量的持续增长，该系统与配套数据集将自动进行更新。